Unc13A dynamically stabilizes vesicle priming at synaptic release sites for short-term facilitation and homeostatic potentiation

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Presynaptic plasticity adjusts neurotransmitter (NT) liberation. Short-term facilitation (STF) tunes synapses to millisecond repetitive activation, while presynaptic homeostatic potentiation (PHP) of NT release stabilizes transmission over minutes. Despite different timescales of STF and PHP, our analysis of Drosophila neuromuscular junctions reveals functional overlap and shared molecular dependence on the release-site protein Unc13A. Mutating Unc13A’s calmodulin binding domain (CaM-domain) increases baseline transmission while blocking STF and PHP. Mathematical modeling suggests that Ca2+/calmodulin/Unc13A interaction plastically stabilizes vesicle priming at release sites and that CaM-domain mutation causes constitutive stabilization, thereby blocking plasticity. Labeling the functionally essential Unc13A MUN domain reveals higher STED microscopy signals closer to release sites following CaM-domain mutation. Acute phorbol ester treatment similarly enhances NT release and blocks STF/PHP in synapses expressing wild-type Unc13A, while CaM-domain mutation occludes this, indicating common downstream effects. Thus, Unc13A regulatory domains integrate signals across timescales to switch release-site participation for synaptic plasticity.
OriginalsprogEngelsk
Artikelnummer112541
TidsskriftCell Reports
Vol/bind42
Udgave nummer6
Antal sider25
ISSN2211-1247
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG) to A.M.W. (Emmy Noether Programme, project 261020751, and the TRR 186, project 278001972) and the Novo Nordisk Foundation (Young Investigator Award NNF19OC0056047 to A.M.W.). M.J. was supported by the Einstein Foundation Berlin (grant EZ-2014-226). We thank Dr. Stephan Sigrist for helpful comments and sharing antibodies. Conceptualization, A.M.W.; methodology, M.J. M.A.B. and J.R.L.K.; formal analysis, M.J. N.B. M.A.B. M.M.M.B. A.E.B. A.B.V. and K.V.P.; investigation, M.J. N.B. M.A.B. M.M.M.B. A.E.B. and A.B.V.; writing – original draft, A.M.W.; writing – review & editing, M.J. N.B. M.M.M.B. A.E.B. A.B.V. K.V.P. and A.M.W.; visualization, M.J. N.B. M.A.B. M.M.M.B. A.E.B. A.B.V. and K.V.P.; supervision, A.M.W.; funding acquisition, A.M.W. M.J. is currently an employee of PPD Germany GmbH & Co. KG (Hansastrasse 32, 80686 Munich, Germany). M.A.B. is currently an employee of Lilly Deutschland GmbH (Werner-Reimers-Straße 2-4, 61352 Bad Homburg, Germany). We support inclusive, diverse, and equitable conduct of research.

Funding Information:
This work was supported by grants from the Deutsche Forschungsgemeinschaft ( DFG ) to A.M.W. (Emmy Noether Programme, project 261020751 , and the TRR 186, project 278001972 ) and the Novo Nordisk Foundation (Young Investigator Award NNF19OC0056047 to A.M.W.). M.J. was supported by the Einstein Foundation Berlin (grant EZ-2014-226 ). We thank Dr. Stephan Sigrist for helpful comments and sharing antibodies.

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© 2023 The Authors

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