TY - JOUR

T1 - Cellular shear stiffness reflects progression of arsenic-induced transformation during G1

AU - Muñoz, Alexandra 

AU - Eldridge, Will J.

AU - Jakobsen, Nina Munkholt

AU - Sørensen, Helle

AU - Wax, Adam

AU - Costa, Max

N1 - Corrigendum: Cellular shear stiffness reflects progression of arsenic-induced transformation during G1 (Carcinogenesis (2018) 39:2 (109-117) - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073581147&doi=10.1093%2fcarcin%2fbgz048&partnerID=40&md5=138bc32037b2a1bc03512640b8fb9c8b

PY - 2018

Y1 - 2018

N2 - Cancer cells consistently exhibit decreased stiffness; however, the onset and progression of this change have not beencharacterized. To study the development of cell stiffness changes, we evaluated the shear stiffness of populations ofcells during transformation to a carcinogenic state. Bronchial epithelial cells were exposed to sodium arsenite to initiateearly stages of transformation. Exposed cells were cultured in soft agar to further transformation and select for clonalpopulations exhibiting anchorage-independent growth. Shear stiffness of various cell populations in G1 was assessed usinga novel non-invasive assay that applies shear stress with fluid flow and evaluates nanoscale deformation using quantitativephase imaging (QPI). Arsenic-treated cells exhibited reduced stiffness relative to control cells, while arsenic clonal lines,selected by growth in soft agar, were found to have reduced stiffness relative to control clonal lines, which were cultured insoft agar but did not receive arsenic treatment. The relative standard deviation (RSD) of the stiffness of Arsenic clones wasreduced compared with control clones, as well as to the arsenic-exposed cell population. Cell stiffness at the populationlevel exhibits potential to be a novel and sensitive framework for identifying the development of cancerous cells.

AB - Cancer cells consistently exhibit decreased stiffness; however, the onset and progression of this change have not beencharacterized. To study the development of cell stiffness changes, we evaluated the shear stiffness of populations ofcells during transformation to a carcinogenic state. Bronchial epithelial cells were exposed to sodium arsenite to initiateearly stages of transformation. Exposed cells were cultured in soft agar to further transformation and select for clonalpopulations exhibiting anchorage-independent growth. Shear stiffness of various cell populations in G1 was assessed usinga novel non-invasive assay that applies shear stress with fluid flow and evaluates nanoscale deformation using quantitativephase imaging (QPI). Arsenic-treated cells exhibited reduced stiffness relative to control cells, while arsenic clonal lines,selected by growth in soft agar, were found to have reduced stiffness relative to control clonal lines, which were cultured insoft agar but did not receive arsenic treatment. The relative standard deviation (RSD) of the stiffness of Arsenic clones wasreduced compared with control clones, as well as to the arsenic-exposed cell population. Cell stiffness at the populationlevel exhibits potential to be a novel and sensitive framework for identifying the development of cancerous cells.

U2 - 10.1093/carcin/bgx116

DO - 10.1093/carcin/bgx116

M3 - Journal article

C2 - 29069374

VL - 39

SP - 109

EP - 117

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 2

ER -