Mid-term function and remodeling potential of tissue engineered tricuspid valve: Histology and biomechanics

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Mid-term function and remodeling potential of tissue engineered tricuspid valve : Histology and biomechanics. / Ropcke, Diana M; Rasmussen, Jonas; Ilkjær, Christine; Skov, Søren N; Tjørnild, Marcell J; Baandrup, Ulrik T; Christian Danielsen, Carl; Hjortdal, Vibeke E; Nielsen, Sten L.

In: Journal of Biomechanics, Vol. 71, 11.04.2018, p. 52-58.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ropcke, DM, Rasmussen, J, Ilkjær, C, Skov, SN, Tjørnild, MJ, Baandrup, UT, Christian Danielsen, C, Hjortdal, VE & Nielsen, SL 2018, 'Mid-term function and remodeling potential of tissue engineered tricuspid valve: Histology and biomechanics', Journal of Biomechanics, vol. 71, pp. 52-58. https://doi.org/10.1016/j.jbiomech.2018.01.019

APA

Ropcke, D. M., Rasmussen, J., Ilkjær, C., Skov, S. N., Tjørnild, M. J., Baandrup, U. T., Christian Danielsen, C., Hjortdal, V. E., & Nielsen, S. L. (2018). Mid-term function and remodeling potential of tissue engineered tricuspid valve: Histology and biomechanics. Journal of Biomechanics, 71, 52-58. https://doi.org/10.1016/j.jbiomech.2018.01.019

Vancouver

Ropcke DM, Rasmussen J, Ilkjær C, Skov SN, Tjørnild MJ, Baandrup UT et al. Mid-term function and remodeling potential of tissue engineered tricuspid valve: Histology and biomechanics. Journal of Biomechanics. 2018 Apr 11;71:52-58. https://doi.org/10.1016/j.jbiomech.2018.01.019

Author

Ropcke, Diana M ; Rasmussen, Jonas ; Ilkjær, Christine ; Skov, Søren N ; Tjørnild, Marcell J ; Baandrup, Ulrik T ; Christian Danielsen, Carl ; Hjortdal, Vibeke E ; Nielsen, Sten L. / Mid-term function and remodeling potential of tissue engineered tricuspid valve : Histology and biomechanics. In: Journal of Biomechanics. 2018 ; Vol. 71. pp. 52-58.

Bibtex

@article{12a293b96a0f44b4a10c54b071d04e36,
title = "Mid-term function and remodeling potential of tissue engineered tricuspid valve: Histology and biomechanics",
abstract = "OBJECTIVE: Tricuspid valve reconstruction using a small intestinal submucosal porcine extracellular matrix (ECM) tube graft is hypothesized to be durable for six months and show signs of recellularization and growth potential. The purpose was to histologically and biomechanically test ECM valves before and after six months of implantation in pigs for comparison with native valves.METHODS: Ten 60 kg pigs were included, which survived tricuspid valve tube graft insertion. Anterior and septal tricuspid leaflets were explanted from all animals surviving more than one month and examined histologically (n = 9). Endothelialization, collagen content, mineralization, neovascularization, burst strength and tensile strength were determined for native valves (n = 5), ECM before implantation (n = 5), and ECM after six months (n = 5).RESULTS: Collagen density was significantly larger in ECM at implantation (baseline) compared to native leaflet tissue (0.3 ± 0.02 mg/mm3 vs. 0.1 ± 0.03 mg/mm3, p < .0001), but collagen density decreased and reached native leaflet collagen content, six months after ECM implantation (native vs. ECM valve at six months: 0.1 ± 0.03 mg/mm3 vs. 0.2 ± 0.05 mg/mm3, p = .8). Histologically, ECM valves showed endothelialization, host cell infiltration and structural collagen organization together with elastin generation after six months, indicating tissue remodeling and -engineering together with gradual development of a close-to-native leaflet structure without foreign body response.CONCLUSIONS: ECM tricuspid tube grafts were stronger than native leaflet tissue. Histologically, the acellular ECM tube grafts showed evidence of constructive tissue remodeling with endothelialization and connective tissue organization. These findings support the concept of tissue engineering and recellularization, which are prerequisites for growth.",
keywords = "Animals, Calcinosis/pathology, Collagen, Elastin, Extracellular Matrix, Heart Valve Prosthesis, Swine, Tensile Strength, Tissue Engineering, Tricuspid Valve",
author = "Ropcke, {Diana M} and Jonas Rasmussen and Christine Ilkj{\ae}r and Skov, {S{\o}ren N} and Tj{\o}rnild, {Marcell J} and Baandrup, {Ulrik T} and {Christian Danielsen}, Carl and Hjortdal, {Vibeke E} and Nielsen, {Sten L}",
note = "Copyright {\textcopyright} 2018 Elsevier Ltd. All rights reserved.",
year = "2018",
month = apr,
day = "11",
doi = "10.1016/j.jbiomech.2018.01.019",
language = "English",
volume = "71",
pages = "52--58",
journal = "Journal of Biomechanics",
issn = "0021-9290",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Mid-term function and remodeling potential of tissue engineered tricuspid valve

T2 - Histology and biomechanics

AU - Ropcke, Diana M

AU - Rasmussen, Jonas

AU - Ilkjær, Christine

AU - Skov, Søren N

AU - Tjørnild, Marcell J

AU - Baandrup, Ulrik T

AU - Christian Danielsen, Carl

AU - Hjortdal, Vibeke E

AU - Nielsen, Sten L

N1 - Copyright © 2018 Elsevier Ltd. All rights reserved.

PY - 2018/4/11

Y1 - 2018/4/11

N2 - OBJECTIVE: Tricuspid valve reconstruction using a small intestinal submucosal porcine extracellular matrix (ECM) tube graft is hypothesized to be durable for six months and show signs of recellularization and growth potential. The purpose was to histologically and biomechanically test ECM valves before and after six months of implantation in pigs for comparison with native valves.METHODS: Ten 60 kg pigs were included, which survived tricuspid valve tube graft insertion. Anterior and septal tricuspid leaflets were explanted from all animals surviving more than one month and examined histologically (n = 9). Endothelialization, collagen content, mineralization, neovascularization, burst strength and tensile strength were determined for native valves (n = 5), ECM before implantation (n = 5), and ECM after six months (n = 5).RESULTS: Collagen density was significantly larger in ECM at implantation (baseline) compared to native leaflet tissue (0.3 ± 0.02 mg/mm3 vs. 0.1 ± 0.03 mg/mm3, p < .0001), but collagen density decreased and reached native leaflet collagen content, six months after ECM implantation (native vs. ECM valve at six months: 0.1 ± 0.03 mg/mm3 vs. 0.2 ± 0.05 mg/mm3, p = .8). Histologically, ECM valves showed endothelialization, host cell infiltration and structural collagen organization together with elastin generation after six months, indicating tissue remodeling and -engineering together with gradual development of a close-to-native leaflet structure without foreign body response.CONCLUSIONS: ECM tricuspid tube grafts were stronger than native leaflet tissue. Histologically, the acellular ECM tube grafts showed evidence of constructive tissue remodeling with endothelialization and connective tissue organization. These findings support the concept of tissue engineering and recellularization, which are prerequisites for growth.

AB - OBJECTIVE: Tricuspid valve reconstruction using a small intestinal submucosal porcine extracellular matrix (ECM) tube graft is hypothesized to be durable for six months and show signs of recellularization and growth potential. The purpose was to histologically and biomechanically test ECM valves before and after six months of implantation in pigs for comparison with native valves.METHODS: Ten 60 kg pigs were included, which survived tricuspid valve tube graft insertion. Anterior and septal tricuspid leaflets were explanted from all animals surviving more than one month and examined histologically (n = 9). Endothelialization, collagen content, mineralization, neovascularization, burst strength and tensile strength were determined for native valves (n = 5), ECM before implantation (n = 5), and ECM after six months (n = 5).RESULTS: Collagen density was significantly larger in ECM at implantation (baseline) compared to native leaflet tissue (0.3 ± 0.02 mg/mm3 vs. 0.1 ± 0.03 mg/mm3, p < .0001), but collagen density decreased and reached native leaflet collagen content, six months after ECM implantation (native vs. ECM valve at six months: 0.1 ± 0.03 mg/mm3 vs. 0.2 ± 0.05 mg/mm3, p = .8). Histologically, ECM valves showed endothelialization, host cell infiltration and structural collagen organization together with elastin generation after six months, indicating tissue remodeling and -engineering together with gradual development of a close-to-native leaflet structure without foreign body response.CONCLUSIONS: ECM tricuspid tube grafts were stronger than native leaflet tissue. Histologically, the acellular ECM tube grafts showed evidence of constructive tissue remodeling with endothelialization and connective tissue organization. These findings support the concept of tissue engineering and recellularization, which are prerequisites for growth.

KW - Animals

KW - Calcinosis/pathology

KW - Collagen

KW - Elastin

KW - Extracellular Matrix

KW - Heart Valve Prosthesis

KW - Swine

KW - Tensile Strength

KW - Tissue Engineering

KW - Tricuspid Valve

U2 - 10.1016/j.jbiomech.2018.01.019

DO - 10.1016/j.jbiomech.2018.01.019

M3 - Journal article

C2 - 29428174

VL - 71

SP - 52

EP - 58

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

ER -

ID: 241757791