Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism
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Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism. / Shah, Shiraz Ali; Hansen, Niels R; Garrett, Roger A.
In: Biochemical Society Transactions, Vol. 37, No. Pt 1, 2009, p. 23-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism
AU - Shah, Shiraz Ali
AU - Hansen, Niels R
AU - Garrett, Roger A
N1 - Key words: acidothermophile, archaeal plasmid, archaeal virus, cluster of regularly interspaced palindromic repeats (CRISPR), crenarchaeon
PY - 2009
Y1 - 2009
N2 - Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches ( approximately 30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.
AB - Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches ( approximately 30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.
U2 - 10.1042/BST0370023
DO - 10.1042/BST0370023
M3 - Journal article
C2 - 19143596
VL - 37
SP - 23
EP - 28
JO - Biochemical Society Transactions
JF - Biochemical Society Transactions
SN - 0300-5127
IS - Pt 1
ER -
ID: 10458137