Pseudohyphal growth in Saccharomyces cerevisiae involves protein kinase-regulated lipid flippases
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Pseudohyphal growth in Saccharomyces cerevisiae involves protein kinase-regulated lipid flippases. / Frøsig, Merethe Mørch; Costa, Sara Rute; Liesche, Johannes; Østerberg, Jeppe Thulin; Hanisch, Susanne; Nintemann, Sebastian; Sørensen, Helle; Palmgren, Michael; Pomorski, Thomas Günther; López-Marqués, Rosa L.
I: Journal of Cell Science, Bind 133, Nr. 15, 235994, 06.08.2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Pseudohyphal growth in Saccharomyces cerevisiae involves protein kinase-regulated lipid flippases
AU - Frøsig, Merethe Mørch
AU - Costa, Sara Rute
AU - Liesche, Johannes
AU - Østerberg, Jeppe Thulin
AU - Hanisch, Susanne
AU - Nintemann, Sebastian
AU - Sørensen, Helle
AU - Palmgren, Michael
AU - Pomorski, Thomas Günther
AU - López-Marqués, Rosa L.
PY - 2020/8/6
Y1 - 2020/8/6
N2 - Lipid flippases of the P4 ATPase family establish phospholipid asymmetry in eukaryotic cell membranes and are involved in many essential cellular processes. The yeast Saccharomyces cerevisiae contains five P4 ATPases, among which Dnf3p is poorly characterized. Here, we demonstrate that Dnf3p is a flippase that catalyzes translocation of major glycerophospholipids, including phosphatidylserine, towards the cytosolic membrane leaflet. Deletion of the genes encoding Dnf3p and the distantly related P4 ATPases Dnf1p and Dnf2p results in yeast mutants with aberrant formation of pseudohyphae, suggesting that the Dnf1p-Dnf3p proteins have partly redundant functions in the control of this specialized form of polarized growth. Furthermore, as previously demonstrated for Dnf1 and Dnf2p, the phospholipid flipping activity of Dnf3p is positively regulated by flippase kinase 1 (Fpk1p) and Fpk2p. Phylogenetic analyses demonstrate that Dnf3p belongs to a subfamily of P4 ATPases specific for fungi and are likely to represent a hallmark of fungal evolution.
AB - Lipid flippases of the P4 ATPase family establish phospholipid asymmetry in eukaryotic cell membranes and are involved in many essential cellular processes. The yeast Saccharomyces cerevisiae contains five P4 ATPases, among which Dnf3p is poorly characterized. Here, we demonstrate that Dnf3p is a flippase that catalyzes translocation of major glycerophospholipids, including phosphatidylserine, towards the cytosolic membrane leaflet. Deletion of the genes encoding Dnf3p and the distantly related P4 ATPases Dnf1p and Dnf2p results in yeast mutants with aberrant formation of pseudohyphae, suggesting that the Dnf1p-Dnf3p proteins have partly redundant functions in the control of this specialized form of polarized growth. Furthermore, as previously demonstrated for Dnf1 and Dnf2p, the phospholipid flipping activity of Dnf3p is positively regulated by flippase kinase 1 (Fpk1p) and Fpk2p. Phylogenetic analyses demonstrate that Dnf3p belongs to a subfamily of P4 ATPases specific for fungi and are likely to represent a hallmark of fungal evolution.
KW - Cell budding
KW - Flippase kinase
KW - Lipid flippase
KW - Polarized growth
KW - Pseudohyphal growth
UR - http://www.scopus.com/inward/record.url?scp=85089300668&partnerID=8YFLogxK
U2 - 10.1242/jcs.235994
DO - 10.1242/jcs.235994
M3 - Journal article
C2 - 32661085
AN - SCOPUS:85089300668
VL - 133
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 15
M1 - 235994
ER -
ID: 247073486