Distribution of RAPD Markers on a Linkage Map of Barley
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Distribution of RAPD Markers on a Linkage Map of Barley. / Giese, H.; Holm‐Jensen, A. G.; Mathiassen, H.; Kjær, B.; Rasmussen, S. K.; Bay, H.; Jensen, J.
I: Hereditas, Bind 120, Nr. 3, 1994, s. 267-273.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Distribution of RAPD Markers on a Linkage Map of Barley
AU - Giese, H.
AU - Holm‐Jensen, A. G.
AU - Mathiassen, H.
AU - Kjær, B.
AU - Rasmussen, S. K.
AU - Bay, H.
AU - Jensen, J.
PY - 1994
Y1 - 1994
N2 - The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.
AB - The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome‐doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.
U2 - 10.1111/j.1601-5223.1994.00267.x
DO - 10.1111/j.1601-5223.1994.00267.x
M3 - Journal article
C2 - 7928390
AN - SCOPUS:0027964749
VL - 120
SP - 267
EP - 273
JO - Hereditas
JF - Hereditas
SN - 0018-0661
IS - 3
ER -
ID: 204470677